Gene name |
SPBC17D11.05 |
Gene ID |
34/A01 |
Gene synonyms/obsolete |
tif32 |
Gene product |
translation initiation
factor (eiF3 p110 subunit); PCI domain |
Entry clone |
Cloned |
ORF length (unspliced) |
2844 |
ORF length (spliced) |
2799 |
Entry clone length |
2844 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
124G:A / 441T:G /
821A:T / 826T:C / 2668T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC17D11.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCACCTCCTCAAGGAAA |
Rev primer name |
SPBC17D11.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTGTTGTTGTTGATTACGT |
Amino acid length |
932 |
Molecular weight |
107 |
Isoelectric point (calc.) |
9.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEVEFHPLSI |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |