Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC27B12.06
Gene ID 33/H11
Gene synonyms/obsolete
Gene product involved in GPI anchor biosynthesis; involved in adding phospho-ethanolamine to the third mannose
Entry clone Cloned
ORF length (unspliced) 2757
ORF length (spliced)
Entry clone length 2757
No. of intron 0
Sequence status Finished
Sequence results 365A:G / 1403T:C / 1867A:G / 2743G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC27B12.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGGAAAAAAGGGTAAA
Rev primer name SPBC27B12.06.Rv
Rev primer SEQ AGAAAGCTGGGTATTCAGCATCTTCAGGAAAT
Amino acid length 918
Molecular weight 104.9
Isoelectric point (calc.) 6.8
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 14
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFPMIIGFLLL/LRTLIVLAL/LSSIILPLIL/LRRVLGLSLKL/LQRPLGHLSL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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