| Gene name |
SPBC2D10.18 |
| Gene ID |
33/E04 |
| Gene synonyms/obsolete |
abc1 |
| Gene product |
involved in assembly
of the ubiquinol cytochrome-c reductase complex (cytochrome
bc1 complex); functionally complements Sc ABC1 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1833 |
| ORF length (spliced) |
|
| Entry clone length |
1833 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
231T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC2D10.18.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTAATTCAGGGCTTTG |
| Rev primer name |
SPBC2D10.18.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCAGCGTAATGTTTCAAC |
| Amino acid length |
610 |
| Molecular weight |
68.5 |
| Isoelectric point (calc.) |
9.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
17 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion;
periphery |
| Comments for localization |
weak signal of
periphery |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |