Gene name |
SPBC543.04 |
Gene ID |
33/E02 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; hypothetical protein; UPF0171 |
Entry clone |
Cloned |
ORF length (unspliced) |
1823 |
ORF length (spliced) |
1758 |
Entry clone length |
1823 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC543.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTACGCTTAACTCCTCG |
Rev primer name |
SPBC543.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACCAAGAATGCCATCGAAGG |
Amino acid length |
585 |
Molecular weight |
66.8 |
Isoelectric point (calc.) |
6.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPSLSTFLAI |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
Microscope used for
observation |
DeltaVision |