Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC8D2.12c
Gene ID 32/G10
Gene synonyms/obsolete pi053
Gene product conserved protein; involved in mitochondrial protein synthesis
Entry clone Cloned
ORF length (unspliced) 1302
ORF length (spliced) 882
Entry clone length 1302
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC8D2.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATGCTTTAAATAAAAT
Rev primer name SPBC8D2.12.Rv
Rev primer SEQ AGAAAGCTGGGTATTCATCAAGAATAGGCAAA
Amino acid length 293
Molecular weight 32.9
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.