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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1556.07
Gene ID 32/C09
Gene synonyms/obsolete pmm1
Gene product phosphomannomutase; involved in intracellular protein transport, protein secretion, protein-ER targeting, cell cycle progression
Entry clone Cloned
ORF length (unspliced) 1006
ORF length (spliced) 774
Entry clone length 1006
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1556.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCAGTTATCCCTATTGA
Rev primer name SPAC1556.07.Rv
Rev primer SEQ AGAAAGCTGGGTATATGTTAAAAATTTTCTTG
Amino acid length 257
Molecular weight 29.2
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LHYIADLDI
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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