Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC31G5.08
Gene ID 31/H07
Gene synonyms/obsolete ups1; ups1
Gene product uroporphyrinogen-III synthase
Entry clone Cloned
ORF length (unspliced) 756
ORF length (spliced)
Entry clone length 756
No. of intron 0
Sequence status Finished
Sequence results 509A:G / 514A:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC31G5.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAACTGCTTTGCTATT
Rev primer name SPAC31G5.08.Rv
Rev primer SEQ AGAAAGCTGGGTAACTAGAATTCTCCTCATCA
Amino acid length 251
Molecular weight 28.5
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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