Gene name |
SPBC21B10.04c |
Gene ID |
31/C09 |
Gene synonyms/obsolete |
nrf1 |
Gene product |
negative regulator of
cdc42p; involved in endocytosis, vacuolar polyphosphate
accumulation, V-ATPase distribution, vacuolar membrane protein
distribution, and protein complex assembly |
Entry clone |
Cloned |
ORF length (unspliced) |
369 |
ORF length (spliced) |
|
Entry clone length |
369 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
147T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC21B10.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTACTCAACCTCTTTT |
Rev primer name |
SPBC21B10.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAATAACATACGAAGAACA |
Amino acid length |
122 |
Molecular weight |
13.3 |
Isoelectric point (calc.) |
11.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
3 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |