Gene name |
SPBC215.04 |
Gene ID |
31/C05 |
Gene synonyms/obsolete |
git11 |
Gene product |
G gamma subunit of the
gpa2/git5/git11 heterotrimeric G protein; no apparent
orthologs; glucose/cAMP pathway; related to Sc YJR086W but not
functional ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
308 |
ORF length (spliced) |
219 |
Entry clone length |
308 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC215.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAACAGAGGCTTTATT |
Rev primer name |
SPBC215.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGAAATAGTACAGCATTTG |
Amino acid length |
72 |
Molecular weight |
7.9 |
Isoelectric point (calc.) |
9.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |