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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC162.08c
Gene ID 31/B06
Gene synonyms/obsolete
Gene product nuclear pore complex associated; similar to Sp SPAC1486.04c; predicted coiled-coil protein
Entry clone Cloned
ORF length (unspliced) 5514
ORF length (spliced)
Entry clone length 5514
No. of intron 0
Sequence status Finished
Sequence results 434A:G / 2195T:C / 4386T:C / 4430A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC162.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCACGATTCTTCCTGGAC
Rev primer name SPCC162.08.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTGCTTTCTTTTGGTTC
Amino acid length 1837
Molecular weight 211.4
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LNDLEKSLVL/LQRTVRNQLEI/LQSTVDSLQL
Localization (YFP) nucleus; nuclear envelope
Comments for localization large nuclear dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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