| Gene name |
SPAC3G9.12 |
| Gene ID |
30/H09 |
| Gene synonyms/obsolete |
|
| Gene product |
CLASP family
microtubule-associated protein Cls1; HEAT repeat |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4510 |
| ORF length (spliced) |
4389 |
| Entry clone length |
4510 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
226T:A / 792T:C /
1272A:G / 2069A:G / 2380A:G / 2565T:C / 4323A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC3G9.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGGATAAGGATGCGCA |
| Rev primer name |
SPAC3G9.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTCTTTTCATCAGACTTC |
| Amino acid length |
1462 |
| Molecular weight |
164 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTTVNPLLL/LQSLVEELSL/LPGILLALLSL/LKSNIDPFLEL/LEPSVLKQLHL |
| Localization (YFP) |
nucleus>>cytosol; nuclear envelope;
microtubules?; SPB? |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |