| Gene name |
SPCC1020.02 |
| Gene ID |
30/F05 |
| Gene synonyms/obsolete |
spc7 |
| Gene product |
hypothetical
protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4095 |
| ORF length (spliced) |
|
| Entry clone length |
4095 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
299T:C / 3707T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1020.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAACATCGCCTCGTCG |
| Rev primer name |
SPCC1020.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGCTGTATTTGTTCCAAACA |
| Amino acid length |
1364 |
| Molecular weight |
153.5 |
| Isoelectric point (calc.) |
4.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMQGIKHELNL |
| Localization (YFP) |
SPB;
nucleus>cytosol; periphery at site of septum
formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |