| Gene name |
SPBC25H2.11c |
| Gene ID |
30/D05 |
| Gene synonyms/obsolete |
|
| Gene product |
bromodomain protein;
involved in transcriptional activation; SAGA complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3342 |
| ORF length (spliced) |
2940 |
| Entry clone length |
3342 |
| No. of intron |
7 |
| Sequence status |
Finished |
| Sequence results |
2553C:T /
2743T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC25H2.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGACTAATTTCGAAGA |
| Rev primer name |
SPBC25H2.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTTCAGGAAGAGTAGTT |
| Amino acid length |
979 |
| Molecular weight |
111.5 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
944 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDSLSVPLHL |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |