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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC23E6.09
Gene ID 30/D01
Gene synonyms/obsolete ssn6
Gene product TPR repeat protein; general repressor of RNA polymerase II transcription
Entry clone Cloned in 2004 trial
ORF length (unspliced) 3309
ORF length (spliced)
Entry clone length 3309
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC23E6.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCCCAATCACAAGTCGC
Rev primer name SPBC23E6.09.Rv
Rev primer SEQ AGAAAGCTGGGTAAACTGACACGGTTTCTTTT
Amino acid length 1102
Molecular weight 121.5
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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