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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC26F1.08c
Gene ID 29/G09
Gene synonyms/obsolete
Gene product conserved hypothetical; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 2977
ORF length (spliced) 2934
Entry clone length 2977
No. of intron 1
Sequence status Finished
Sequence results 568A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC26F1.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATTTCCACCATGGCTG
Rev primer name SPAC26F1.08.Rv
Rev primer SEQ AGAAAGCTGGGTACGCCATTTTCTTCCCAATC
Amino acid length 977
Molecular weight 111.3
Isoelectric point (calc.) 9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 12
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSRNLYTNLTI/LIIKLENLGI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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