Gene name |
SPBC16G5.16 |
Gene ID |
29/F07 |
Gene synonyms/obsolete |
|
Gene product |
transcriptional
regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6)
binuclear cluster domain |
Entry clone |
Cloned |
ORF length (unspliced) |
2861 |
ORF length (spliced) |
2484 |
Entry clone length |
2861 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
33T:C / 1787A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16G5.16.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTCCGGCGATGTATC |
Rev primer name |
SPBC16G5.16.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCGGTTCTTTTAGAGTTT |
Amino acid length |
827 |
Molecular weight |
94.5 |
Isoelectric point (calc.) |
7.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
cytoplasmic dots by
over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |