Chemical Genetics Laboratory
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC57A7.10c
Gene ID 29/E05
Gene synonyms/obsolete sec21
Gene product adaptin; coatomer (gamma subunit); the coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network; coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins (By similarity); the COPG family
Entry clone Cloned
ORF length (unspliced) 2821
ORF length (spliced) 2718
Entry clone length 2821
No. of intron 2
Sequence status Finished
Sequence results 4A:G / 1856T:C / 2489T:C
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC57A7.10.Fd
Rev primer name SPAC57A7.10.Rv
Amino acid length 905
Molecular weight 100.9
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LQPVVSVLKI/LPALERSLVI
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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