Gene name |
SPAC3G6.09c |
Gene ID |
29/D02 |
Gene synonyms/obsolete |
|
Gene product |
glycosyl transferase
family 20; trehalose-phosphate synthase; similar to Sp TPP1
|
Entry clone |
Cloned |
ORF length (unspliced) |
2550 |
ORF length (spliced) |
|
Entry clone length |
2550 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
499A:G / 896A:G /
2210T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC3G6.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTTCAGGCGCGCAAGG |
Rev primer name |
SPAC3G6.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAGGTTATGCAAAAGTTCT |
Amino acid length |
849 |
Molecular weight |
96.3 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRAFRELLI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |