Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3G6.09c
Gene ID 29/D02
Gene synonyms/obsolete
Gene product glycosyl transferase family 20; trehalose-phosphate synthase; similar to Sp TPP1
Entry clone Cloned
ORF length (unspliced) 2550
ORF length (spliced)
Entry clone length 2550
No. of intron 0
Sequence status Finished
Sequence results 499A:G / 896A:G / 2210T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3G6.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTTCAGGCGCGCAAGG
Rev primer name SPAC3G6.09.Rv
Rev primer SEQ AGAAAGCTGGGTATAGGTTATGCAAAAGTTCT
Amino acid length 849
Molecular weight 96.3
Isoelectric point (calc.) 7.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRAFRELLI
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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