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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC14C4.05c
Gene ID 29/C08
Gene synonyms/obsolete
Gene product conserved hypothetical; hypothetical protein; ATP/GTP-binding site motif
Entry clone Cloned
ORF length (unspliced) 2535
ORF length (spliced)
Entry clone length 2535
No. of intron 0
Sequence status Finished
Sequence results 1176A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC14C4.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGGTCCCTTCTTATTT
Rev primer name SPAC14C4.05.Rv
Rev primer SEQ AGAAAGCTGGGTATAAGGCAATGTCATCTGAA
Amino acid length 844
Molecular weight 94.9
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFSPLFTLEL/LFELVHLPLSI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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