| Gene name |
SPBC1685.14c |
| Gene ID |
29/C04 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; hypothetical protein; non-essential |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
2527 |
| ORF length (spliced) |
2406 |
| Entry clone length |
2527 |
| No. of intron |
2 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC1685.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTATGCTAAAATCATT |
| Rev primer name |
SPBC1685.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATATGGTGCATTGACAATG |
| Amino acid length |
801 |
| Molecular weight |
92.4 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LENIVTKLDL/LMGTISAELHL |
| Localization (YFP) |
cytosol; a few
cytoplasmic dots; nuclear envelope? |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |