| Gene name |
SPAC3F10.02c |
| Gene ID |
29/C02 |
| Gene synonyms/obsolete |
trk1 |
| Gene product |
potassium transporter;
TrkH domain; similar to Sp trk2 (paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2526 |
| ORF length (spliced) |
|
| Entry clone length |
2526 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC3F10.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTCCTAAATTACATTCA |
| Rev primer name |
SPAC3F10.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTCTCGAGAAACTGCAATA |
| Amino acid length |
841 |
| Molecular weight |
96 |
| Isoelectric point (calc.) |
9.6 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
8 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLLISSLFI/LLLNVIDLVL/LWYIFLGLFI |
| Localization (YFP) |
Golgi?; periphery at
cell tip and site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |