| Gene name |
SPAC16E8.07c |
| Gene ID |
28/H06 |
| Gene synonyms/obsolete |
vph1 |
| Gene product |
vacuolar ATP synthase
subunit; V-type ATPase subunit; vacuolar proton pump
component; V0 sector |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2471 |
| ORF length (spliced) |
2418 |
| Entry clone length |
2471 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
17A:G / 202A:G /
703A:G / 2378T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC16E8.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGGCACTTGGGGAGTT |
| Rev primer name |
SPAC16E8.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCCGCGGTCACTTTGAAA |
| Amino acid length |
805 |
| Molecular weight |
91.2 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
6 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LWRTLRGNLFI/LEAIFGYLVI/LICVPWLLI |
| Localization (YFP) |
ER? |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |