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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC825.01
Gene ID 28/H04
Gene synonyms/obsolete
Gene product ABC transporter; unknown specificity
Entry clone Cloned
ORF length (unspliced) 2469
ORF length (spliced)
Entry clone length 2469
No. of intron 0
Sequence status Finished
Sequence results 570T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC825.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTAGGGGAAAGCGTTC
Rev primer name SPCC825.01.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTTTTTGCTTCTCCAAT
Amino acid length 822
Molecular weight 92.6
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 179/190/151
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LADDMDDLSL/LTCVEAVLDI
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: nucleolus>nucleus>cytosol
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images
LMB 4 images) See all images

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