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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC29B12.02c
Gene ID 28/F02
Gene synonyms/obsolete
Gene product histone lysine methyltransferase; SET domain; WW domain (inferred from context)
Entry clone Cloned
ORF length (unspliced) 2397
ORF length (spliced)
Entry clone length 2397
No. of intron 0
Sequence status Finished
Sequence results 1497C:T / 1544C:T / 2288G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC29B12.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAGACGGCATCATCTCT
Rev primer name SPAC29B12.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAGCTTTTTTCGGGGAT
Amino acid length 798
Molecular weight 90.6
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 651/588
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LPENVREALGI
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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