| Gene name |
SPAC6G10.07 |
| Gene ID |
28/E10 |
| Gene synonyms/obsolete |
|
| Gene product |
nuclear cap-binding
complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2392 |
| ORF length (spliced) |
2343 |
| Entry clone length |
2392 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1251A:G / 2370A:G /
2371T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC6G10.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTCTTATCGGGGATC |
| Rev primer name |
SPAC6G10.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTTCCTCATTAGTTGCA |
| Amino acid length |
780 |
| Molecular weight |
90 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDLLYKQLIL/LMYTMSSDLPL/LHKPLEELDI |
| Localization (YFP) |
nucleus>>cytosol; spindle microtubules |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |