Gene name |
SPBC83.05 |
Gene ID |
28/C10 |
Gene synonyms/obsolete |
|
Gene product |
RNA-binding protein;
rrm RNA recognition motif; involved in early maturation of
pre-rRNA and mitochondrial maintenance |
Entry clone |
Cloned |
ORF length (unspliced) |
2322 |
ORF length (spliced) |
|
Entry clone length |
2322 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
56A:C / 620T:C /
1447C:T / 1523C:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC83.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTTGTTTCTATCTCG |
Rev primer name |
SPBC83.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTCGATTTTTTTCAAAGCC |
Amino acid length |
773 |
Molecular weight |
88.2 |
Isoelectric point (calc.) |
8.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |