| Gene name |
SPAC7D4.11c |
| Gene ID |
28/C07 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
similar to N. crassa 15E6.130; no apparent Sc ortholog |
| Entry clone |
Cloned/2003.08 |
| ORF length (unspliced) |
2310 |
| ORF length (spliced) |
|
| Entry clone length |
2310 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
664T:C |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC7D4.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATGAAGACAAATTGGA |
| Rev primer name |
SPAC7D4.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTACGAGGGGGAACTCCT |
| Amino acid length |
769 |
| Molecular weight |
87.5 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYLLISLII/LAVVHTLVL/LYDDILALDI |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
ER at low expression
level? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |