| Gene name |
SPBC887.03c |
| Gene ID |
28/C04 |
| Gene synonyms/obsolete |
|
| Gene product |
basic helix-loop-helix
(bHLH); involved in DNA replication; NucleOlar Complex 2;
involved in nuclear export of pre-ribosomes; interacts with
MCM proteins; involved in the establishment and maintenance of
pre-replication complexes; conserved eukaryotic protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2289 |
| ORF length (spliced) |
2244 |
| Entry clone length |
2289 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
648A:G / 1686A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC887.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGGCTCGGAAGAATCA |
| Rev primer name |
SPBC887.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAACGAAGTGCTTTTTAAC |
| Amino acid length |
747 |
| Molecular weight |
85.1 |
| Isoelectric point (calc.) |
8.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
442 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEEPVENLHL/LPDVYDLFL/LSSFSKRLAI |
| Localization (YFP) |
nucleolus>>nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |