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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC20F10.08c
Gene ID 28/A09
Gene synonyms/obsolete
Gene product hypothetical protein
Entry clone Cloned
ORF length (unspliced) 2244
ORF length (spliced)
Entry clone length 2244
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC20F10.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGCAAATCAGAAGTT
Rev primer name SPBC20F10.08.Rv
Rev primer SEQ AGAAAGCTGGGTATTGTAAATAGTATTTCGCT
Amino acid length 747
Molecular weight 84.8
Isoelectric point (calc.) 5.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LAMFLPNLLEI
Localization (YFP) SPB; nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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