| Gene name |
SPAC27D7.13c |
| Gene ID |
27/E02 |
| Gene synonyms/obsolete |
ssm4;
SPAC637.01c |
| Gene product |
p150-Glued;
microtubule-associated protein; expressed during meiosis;
CAP-Gly domain; similar to S. cerevisiae YPL174C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2013 |
| ORF length (spliced) |
|
| Entry clone length |
2013 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC27D7.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTATTTGTCCGTCGG |
| Rev primer name |
SPAC27D7.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGCATTTTTTGGAAAGAATG |
| Amino acid length |
670 |
| Molecular weight |
77.1 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
276/278 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNIVPGSLDL/LTEVKHLKL |
| Localization (YFP) |
microtubules,
especially in meiotic cells |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |