| Gene name |
SPAC343.07 |
| Gene ID |
27/B10 |
| Gene synonyms/obsolete |
|
| Gene product |
RNA-binding protein;
similar to Sp crp79 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1970 |
| ORF length (spliced) |
1830 |
| Entry clone length |
1970 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
32A:G / 478T:C /
1079T:C / 1658A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC343.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTGCACTTGAAAAACT |
| Rev primer name |
SPAC343.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGGTTTAATACAATGTTG |
| Amino acid length |
609 |
| Molecular weight |
68.5 |
| Isoelectric point (calc.) |
7.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
488 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |