Gene name |
SPAC6G9.02c |
Gene ID |
27/B07 |
Gene synonyms/obsolete |
|
Gene product |
RNA-binding protein;
pumilio family |
Entry clone |
Cloned |
ORF length (unspliced) |
1968 |
ORF length (spliced) |
|
Entry clone length |
1968 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6G9.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAAAAAAAAGAAAAAC |
Rev primer name |
SPAC6G9.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGACTTTCACTCTTTTAGGA |
Amino acid length |
655 |
Molecular weight |
75.4 |
Isoelectric point (calc.) |
7.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSHMAAILNL |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|