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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1322.08
Gene ID 27/B03
Gene synonyms/obsolete srk1
Gene product MAPK-activated protein kinase; involved in osmotic stress; meiotic regulator; interacts physically with Sty1p
Entry clone Cloned
ORF length (unspliced) 1966
ORF length (spliced) 1743
Entry clone length 1966
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1322.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGTTTTAAAGTATGTTT
Rev primer name SPCC1322.08.Rv
Rev primer SEQ AGAAAGCTGGGTACACTTTTTGTCGATGTCGA
Amino acid length 580
Molecular weight 66.1
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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