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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC297.03
Gene ID 27/A11
Gene synonyms/obsolete ssp1
Gene product serine/threonine protein kinase; deletion mutant sensitive to multiple stresses; acts in a different pathway to Spc1p
Entry clone Cloned
ORF length (unspliced) 1959
ORF length (spliced)
Entry clone length 1959
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC297.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGATCAGTGAATAATGA
Rev primer name SPCC297.03.Rv
Rev primer SEQ AGAAAGCTGGGTAATTAGTTGGTGTGAAGGAA
Amino acid length 652
Molecular weight 73.9
Isoelectric point (calc.) 7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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