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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC11D3.07c
Gene ID 26/F11
Gene synonyms/obsolete
Gene product involved in transcriptional regulation; zinc finger protein; zf-fungal Zn(2)-Cys(6) binuclear cluster domain; similar to Sp SPAPB2C8.02 and SPBC1773.16C and SPBC1773.12 and SPBC530.05 and SPBC530.08 (paralogs)
Entry clone Cloned
ORF length (unspliced) 1914
ORF length (spliced) 1812
Entry clone length 1914
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC11D3.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTCAAAATAAAGCATG
Rev primer name SPAC11D3.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGATTCCTCATAACATTG
Amino acid length 603
Molecular weight 69.8
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LEEKIDLLLDI/LISFYSILAL/LYCPFTPFLII
Localization (YFP) nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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