| Gene name |
SPCC962.03c |
| Gene ID |
26/A09 |
| Gene synonyms/obsolete |
cut15 |
| Gene product |
alpha-importin/karyopherin; serine rich RNA
polymerase I suppressor; involved in mitosis; involved in
chromosome condensation (required); armadillo repeat protein;
similar to Sp SPBC1604.08C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1629 |
| ORF length (spliced) |
|
| Entry clone length |
1629 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1116A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC962.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGCATCTTCTCGCTT |
| Rev primer name |
SPCC962.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACATAGCCATATCTTCGGCA |
| Amino acid length |
542 |
| Molecular weight |
60.4 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear envelope |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |