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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC27E2.01
Gene ID 26/A07
Gene synonyms/obsolete
Gene product alpha-amylase; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1629
ORF length (spliced) 1476
Entry clone length 1629
No. of intron 2
Sequence status Finished
Sequence results 933T:A / 1156C:T / 1277G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC27E2.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTCTAGATAAACAAAT
Rev primer name SPAC27E2.01.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCCAGTTCATTCATGGAA
Amino acid length 491
Molecular weight 56.6
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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