| Gene name |
SPAC4D7.08c |
| Gene ID |
25/F11 |
| Gene synonyms/obsolete |
ade4; min13;
aza1 |
| Gene product |
amidophosphoribosyltransferase; glutamine
phosphoribosylpyrophosphate amidotransferase; involved in
purine biosynthesis; purine nucleotide de-novo biosynthetic
pathway; involved in glutamate metabolism; expression not
repressed by ade4 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1602 |
| ORF length (spliced) |
|
| Entry clone length |
1602 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1426T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4D7.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGCGGAATTTTGGCGTT |
| Rev primer name |
SPAC4D7.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACAAAATCAAAAGTAACA |
| Amino acid length |
533 |
| Molecular weight |
59.8 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytoplasmic dots;
faint filamentous structures |
| Comments for localization |
bright dots by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |