Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC6G10.12c
Gene ID 25/F09
Gene synonyms/obsolete
Gene product zinc finger protein; zf-C2H2 type (3); regulator of eng1; possibly involved in metallothionein expression; deletion mutant displays severe cell separation defects; involved in cytokinesis; involved in septation; involved in cell separation (regulation)
Entry clone Cloned (Re-cloned)
ORF length (unspliced) 1602
ORF length (spliced)
Entry clone length 1602
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC6G10.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGCTTTCATATTTATC
Rev primer name SPAC6G10.12.Rv
Rev primer SEQ AGAAAGCTGGGTAGTGCTGTCTGCGATCTACG
Amino acid length 533
Molecular weight 59.8
Isoelectric point (calc.) 5.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) almost no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation DeltaVision

Image information
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