| Gene name |
SPAC4D7.10c |
| Gene ID |
25/F03 |
| Gene synonyms/obsolete |
|
| Gene product |
sequence orphan;
hypothetical protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1591 |
| ORF length (spliced) |
1422 |
| Entry clone length |
1591 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1242A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4D7.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAGAAATAATGGTTT |
| Rev primer name |
SPAC4D7.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCTTTCAGCCTTCATTTGC |
| Amino acid length |
473 |
| Molecular weight |
54 |
| Isoelectric point (calc.) |
9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
278 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIKIRQLAI |
| Localization (YFP) |
nuclear dots;
cytoplasmic dots; nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |