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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC965.10
Gene ID 25/D08
Gene synonyms/obsolete
Gene product transcriptional regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6) binuclear cluster domain
Entry clone Cloned
ORF length (unspliced) 1578
ORF length (spliced)
Entry clone length 1578
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC965.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACATCGACATCTCATTT
Rev primer name SPCC965.10.Rv
Rev primer SEQ AGAAAGCTGGGTATACACCAGTATTTAACCTC
Amino acid length 525
Molecular weight 59.8
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LENCIKPLVL
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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