| Gene name |
SPCC338.15 |
| Gene ID |
25/D06 |
| Gene synonyms/obsolete |
|
| Gene product |
dolichyl-di-phosphooligosaccharide-protein
glycotransferase subunit; oligosaccharyltransferase; involved
in N-linked glycosylation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1577 |
| ORF length (spliced) |
1314 |
| Entry clone length |
1577 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
1500T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC338.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGAGCGCATTGTGTAT |
| Rev primer name |
SPCC338.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTTGTTTTTTGGCAGAA |
| Amino acid length |
437 |
| Molecular weight |
48.8 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |