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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC757.13
Gene ID 25/C11
Gene synonyms/obsolete
Gene product transporter; unknown specificity; allantoate permease family; similar to Sp SPCC417.10 and SPBC1773.15
Entry clone Cloned
ORF length (unspliced) 1569
ORF length (spliced)
Entry clone length 1569
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC757.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTCCATCACTTCAAG
Rev primer name SPCC757.13.Rv
Rev primer SEQ AGAAAGCTGGGTACCATCTATAACGAAATTTG
Amino acid length 522
Molecular weight 58.6
Isoelectric point (calc.) 8.9
Signal SEQ
No. of transmembrane domain 12
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LIGVFMNCLTL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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