| Gene name |
SPBC16H5.05c |
| Gene ID |
25/C03 |
| Gene synonyms/obsolete |
cyp7; cwf27 |
| Gene product |
cyclophilin;
peptidyl-prolyl cis-trans isomerase; complexed with Cdc5p;
involved in mRNA splicing; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1564 |
| ORF length (spliced) |
1392 |
| Entry clone length |
1564 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
169G:A / 1161T:A /
1458A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC16H5.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCGTAAGTATATTTTT |
| Rev primer name |
SPBC16H5.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATTATATCGCGTTTCATT |
| Amino acid length |
463 |
| Molecular weight |
52.1 |
| Isoelectric point (calc.) |
8.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |