| Gene name |
SPAC227.07c |
| Gene ID |
25/B10 |
| Gene synonyms/obsolete |
pab1 |
| Gene product |
protein phosphatase
pp2a regulatory subunit b; involved in cellular morphogenesis;
involved in cell wall biosynthesis; involved in cytokinesis;
WD repeat protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1556 |
| ORF length (spliced) |
1392 |
| Entry clone length |
1556 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
472G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC227.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGATATAGAAGACTC |
| Rev primer name |
SPAC227.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGAGCTTAGAGAAAACAAAA |
| Amino acid length |
463 |
| Molecular weight |
52.7 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRINLWNLSI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |