| Gene name |
SPBC661.01 |
| Gene ID |
25/B05 |
| Gene synonyms/obsolete |
SPBC530.15c |
| Gene product |
phosphatidylserine
decarboxylase; involved in phosphatidylcholine biosynthesis;
phosphatidylserine decarboxylase activity; localization
mitochondrial inner membrane; similar to Sp SPBC16E9.18 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1551 |
| ORF length (spliced) |
|
| Entry clone length |
1551 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
74A:G / 596T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC661.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCGAACGTACTTCAGA |
| Rev primer name |
SPBC661.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCCTTTAGACAGTGCTTG |
| Amino acid length |
516 |
| Molecular weight |
57.3 |
| Isoelectric point (calc.) |
8.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIPVPFMLFL |
| Localization (YFP) |
periphery |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |