Gene name |
SPAC19B12.10 |
Gene ID |
25/A12 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; similar to human amsh protein; MOV34 domain; no
apparent Sc ortholog |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1547 |
ORF length (spliced) |
1308 |
Entry clone length |
1547 |
No. of intron |
3 |
Sequence status |
Partially
sequenced |
Sequence results |
100% match in both
ends |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC19B12.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACATTCTCCAAGGATC |
Rev primer name |
SPAC19B12.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTAACCCTCAAATCAACA |
Amino acid length |
435 |
Molecular weight |
49.3 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no expression clone
|
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
|