Gene name |
SPAC4A8.01 |
Gene ID |
25/A03 |
Gene synonyms/obsolete |
mde5; meu30;
SPAC25H1.09 |
Gene product |
alpha-amylase; meiotic
expression upregulated; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1542 |
ORF length (spliced) |
|
Entry clone length |
1542 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1098T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4A8.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGCACAACGAAGTTTT |
Rev primer name |
SPAC4A8.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTTCCTATCGGGCCCATTA |
Amino acid length |
513 |
Molecular weight |
58.7 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |