Gene name |
SPBC12C2.06 |
Gene ID |
24/E05 |
Gene synonyms/obsolete |
|
Gene product |
DEAD/DEAH box
helicase; ATP-dependent; RNA helicase; involved in mRNA expor
|
Entry clone |
Cloned |
ORF length (unspliced) |
1512 |
ORF length (spliced) |
|
Entry clone length |
1512 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC12C2.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAACTACACTGGGACA |
Rev primer name |
SPBC12C2.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATTTTAAGTGCATTCTTG |
Amino acid length |
503 |
Molecular weight |
56.3 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVELYGLLTI |
Localization (YFP) |
cytosol; nuclear
envelope |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol; nuclear envelope |
Microscope used for
observation |
Leica |