Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPCC330.07c
Gene ID 24/D04
Gene synonyms/obsolete
Gene product transporter; unknown specificity; no apparent Sc ortholog; (old-> MFS efflux transporter of unknown specificity)
Entry clone Cloned
ORF length (unspliced) 1503
ORF length (spliced)
Entry clone length 1503
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC330.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGCCATCACAACGAAG
Rev primer name SPCC330.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATGTGTTTTGCATTTCAAG
Amino acid length 500
Molecular weight 56.1
Isoelectric point (calc.) 9.5
Signal SEQ
No. of transmembrane domain 12
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFMWFFYMLTI
Localization (YFP) Golgi; periphery at cell tip and site of septum formation
Comments for localization accumulated Golgi by over expression
Effect of LMB on protein localization no change
Microscope used for observation Confocal, DeltaVision

Image information
YFP 3 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.